Summary
Type: SPE
Application #: 301100
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
9 ml methanol, 6 ml water, 2 ml phosphate buffer pH 7.6
Sample application:
Slowly force or aspirate the sample through the column
Column washing:
9 ml dist. water, then 6 ml water / methanol (4 : 1, v/v), finally 500 μl
methanol; then dry column under vacuum
Elution:
9 ml methanol; concentrate eluate in a stream of nitrogen at 60 °C
Authors: Macherey-Nagel
Source: LC Department